Lupus anticoagulant testing using plasma spiked with monoclonal antibodies: performance in the UKNEQAS proficiency testing programme
(2004) Journal of Thrombosis and Haemostasis — Vol. 2, n° 12, p. 2178-2184 (2004)
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- Abstract
- We report here results from a United Kingdom National Quality Assessment Scheme (UK NEQAS) exercise in which both plasma spiked with monoclonal antibodies and plasma from a patient known to have lupus anticoagulant (LA) were distributed to 245 hemostasis laboratories with a request for them to test for possible LA using their routine screening procedure. In general, good agreement was seen in the diagnosis of samples spiked with monoclonal antibodies against beta2-glycoprotein 1 (beta2GP1) and prothrombin, the LA-positive patient sample, and a normal pooled plasma; over 87% of centers correctly identified each sample. However, methods employing platelet neutralizing procedures were associated with a higher proportion of false-negative responses with the antiprothrombin-spiked sample, and it is important to recognize that sensitivity and responsiveness of different methods may vary between artificial plasmas and different LA-positive patient plasmas.
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Jennings, I., Mackie, I., Arnout, J., & Preston, F. (2004). Lupus anticoagulant testing using plasma spiked with monoclonal antibodies: performance in the UKNEQAS proficiency testing programme. Journal of Thrombosis and Haemostasis, 2(12), 2178-2184. https://doi.org/10.1111/j.1538-7836.2004.01028.x (Original work published 2004)