Unraveling the Effect on Luminescent Properties by Postsynthetic Covalent and Noncovalent Grafting of gfp Chromophore Analogues in Nanoscale MOF-808

Singh, Ashish;Karmakar, Sanchita;Abraham, Irine Maria;Darsi Rambabu;Maji, Tapas Kumar;et.al.
(2020) Inorganic Chemistry — Vol. 59, n° 12, p. 8251-8258 (2020)

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Authors
  • Singh, Ashishorcid-logo
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  • Karmakar, Sanchita
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  • Abraham, Irine Maria
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  • Darsi Rambabuorcid-logoUCLouvain
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  • Maji, Tapas Kumarorcid-logo
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Abstract
Here, we demonstrate mimicking of photophysical properties of native green fluorescent protein (gfp) by immobilizing the gfp chromophore analogues in nanoscale MOF-808 and further exploring the bioimaging applications. The two virtually nonfluorescent gfp chromophore analogues carrying different functionalities, BDI-AE (COOH/COOMe) and BDI-EE (COOMe/COOMe) were immobilized in nanosized MOF-808 via postsynthetic modification. An 1H NMR and IR study confirms that BDI-AE was coordinated in NMOF-808, whereas BDI-EE was just noncovalently encapsulated. Interestingly, the extremely weakly fluorescent monomers BDI-AE and BDI-EE (QY = 0.01–0.03%, lifetime = 0.01–0.03 ns) showed a 102-fold increase in quantum efficiency with a significantly longer excited-state lifetime (QY = 1.8–5.6%, lifetime 0.89–1.49 ns) after immobilization in the NMOF-808 scaffold. Moreover, BDI-AE@MOF-808 has 4 times higher quantum efficiency as well as longer excited-state lifetime in comparison to BDI-EE@NMOF-808 due to the rigidity imposed in the chromophore upon coordination with Zr4+ in the former case. Further, a cell viability test performed for BDI-AE@NMOF-808 in HeLa cells confirmed the nontoxic nature of the material and, more importantly, bioimaging applications have also been explored successfully.
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  • JNCASRIndia

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Singh, A., Karmakar, S., Abraham, I. M., Darsi Rambabu, Dave, D., Manjithaya, R., & Maji, T. K. (2020). Unraveling the Effect on Luminescent Properties by Postsynthetic Covalent and Noncovalent Grafting of gfp Chromophore Analogues in Nanoscale MOF-808. Inorganic Chemistry, 59(12), 8251-8258. https://doi.org/10.1021/acs.inorgchem.0c00625 (Original work published 2020)