The effect of low temperature atmospheric nitrogen plasma on MC3T3-E1 preosteoblast proliferation and differentiation in vitro

Przekora, Agata;Pawlat, Joanna;Terebun, Piotr;Duday, David;Ginalska, Grazyna;et.al.
(2019) Journal of Physics D: Applied Physics — Vol. 52, n° 27, p. 275401 (2019)

Files

Przekora_2019_J_Phys_D__Appl_Phys_52_275401.pdf
  • Open Access
  • Adobe PDF
  • 2.43 MB
Download

Details

Authors
  • Przekora, Agataorcid-logoChair and Department of Biochemistry and Biotechnology, Medical University of Lublin, Lublin, Poland
    Author
  • Pawlat, JoannaInstitute of Electrical Engineering and Electrotechnologies, Lublin University of Technology, Lublin, Poland
    Author
  • Terebun, PiotrInstitute of Electrical Engineering and Electrotechnologies, Lublin University of Technology, Lublin, Poland
    Author
  • Duday, DavidMaterial Research and Technology (MRT) Department, Luxembourg Institute of Science and Technology (LIST), Belvaux, Luxembourg
    Author
  • Hermans, SophieUCLouvain
    Author
  • Audemar, MaïtéUCLouvain
    Author
  • Ginalska, GrazynaChair and Department of Biochemistry and Biotechnology, Medical University of Lublin, Lublin, Poland
    Author
Show more
Abstract
The aim of this work was to evaluate the impact of atmospheric pressure nitrogen plasma on viability, proliferation, and osteogenic differentiation of normal mouse calvarial preosteoblasts (MC3T3-E1 Subclone 4), which were maintained in Hanks’ balanced salt solution (HBSS)during plasma exposure. Obtained results clearly demonstrated that short-time (4, 8, and 16 s) nitrogen plasma treatment is non-toxic to the MC3T3-E1 cells, does not affect cell morphology, promotes reosteoblasts’ proliferation, enhances osteogenic differentiation by increasing bone alkaline phosphatase and osteocalcin concentration, but inhibits mineralization of extracellular matrix. The best results were achieved for 16 s exposure time and when the preosteoblasts were left in HBSS for 3 h after plasma treatment. Presented studies indicate great clinical potential of cold atmospheric nitrogen plasma for regenerative medicine applications to improve bone healing process.
Affiliations

Citations

  • APA
  • Chicago
  • FWB

Przekora, A., Pawlat, J., Terebun, P., Duday, D., Canal, C., Hermans, S., Audemar, M., Labay, C., Thomann, J.-S., & Ginalska, G. (2019). The effect of low temperature atmospheric nitrogen plasma on MC3T3-E1 preosteoblast proliferation and differentiation in vitro. Journal of Physics D: Applied Physics, 52(27), 275401. https://doi.org/10.1088/1361-6463/ab1a02 (Original work published 2019)