Identification of Protein Partners by APEX2 Proximity Labeling.
(2024) Bacterial Secretion Systems : Methods and Protocols — ISBN: [9781071634455], p. 321–329, published
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- Abstract
- Proximity labeling methods enable the identification of proteins in the vicinity of a protein of interest in living cells. Among them, APEX2 proximity is a powerful method to spatiotemporally define in vivo "proxisomes" in dynamic bacterial protein systems. Here we describe a standardized APEX2 proximity labeling protocol and possible adaptations to capture protein partners in native conditions.
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Remy, O., & Santin, Y. (2024). Identification of Protein Partners by APEX2 Proximity Labeling. In Laure Journet, Eric Cascales (ed.), Bacterial Secretion Systems : Methods and Protocols (2nd edition, p. p. 321–329). Humana Press. https://doi.org/10.1007/978-1-0716-3445-5_21