Fructose administration stimulates glucose phosphorylation in the livers of anesthetized rats.
(1991) The FASEB journal : official publication of the Federation of American Societies for Experimental Biology — Vol. 5, n° 3, p. 326-330 (1991)
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- Abstract
- A method allowing one to measure the rate of glucose phosphorylation in the livers of anesthetized rats is described. Upon injection of [2-3H]glucose into the portal vein, about 90% of the radioactivity remained in the liver for approximately 30 s. The proportion of radioactivity accounted for by tritiated water increased linearly at a rate of about 5%/min in control animals. Fructose injected into the penile vein stimulated this rate up to 2.2- and 2.7-fold in fed and overnight starved rats respectively, a maximal effect being observed at a dose of 50 mg/kg under both conditions. Fructose was also active when administered by intragastric infusion. The ketose caused increases in the concentration of fructose 1-phosphate, which reached values known to relieve the inhibition exerted on glucokinase by its regulatory protein.
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Citations
Van Schaftingen, E., & Davies, D. R. (1991). Fructose administration stimulates glucose phosphorylation in the livers of anesthetized rats. The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 5(3), 326-330. https://hdl.handle.net/2078.5/80108 (Original work published 1991)