Liquid chromatographic separation of hexopyranosylated cytosine nucleosides from their degradation products
(1997) Journal of Pharmaceutical and Biomedical Analysis — Vol. 16, n° 3, p. 533-540 (1997)
Files
pdfdocument.pdf
Restricted Access - Adobe PDF
- 129.58 KB
Details
- Authors
- Abstract
- Development of a liquid chromatographic method which can separate each of a series of hexopyranosylated cytosine nucleosides from their degradation products formed at acid, neutral and basic pH is described. Both silica-based reverse-phase and polymer columns were examined. Influence of the mobile phase pH, ion-pairing agent, concentration of the buffer and type and concentration of organic modifier were systematically investigated. The concentration of the ion-pairing agent and the buffer were found to have a major effect on selectivity. Samples were finally analyzed on a poly(styrene-divinylbenzene), PLRP-S 100 Angstrom (8 mu m) 250 x 4.6 mm I.D. column at 60 degrees C and with a mobile phase consisting of acetonitrile-sodium octanesulphonate (pH 2.5; 0.02 M)-potassium phosphate buffer (pH 2.5; 0.2 M)-water (X:25:50:25 -X, v/v, where X is variable). (C) 1997 Elsevier Science B.V.
- Affiliations
Citations
Thoithi, G., Vanschepdael, A., Herdewijn, P., Roets, E., & Hoogmartens, J. (1997). Liquid chromatographic separation of hexopyranosylated cytosine nucleosides from their degradation products. Journal of Pharmaceutical and Biomedical Analysis, 16(3), 533-540. https://doi.org/10.1016/S0731-7085(97)00100-3 (Original work published 1997)