Lactococcus lactis as a cell factory for high-level diacetyl production
(2000) Applied and Environmental Microbiology — Vol. 66, n° 9, p. 4112-4114 (2000)
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- Abstract
- We report the engineering of Lactococcus lactis for the efficient conversion of sugar into diacetyl by combining NADH-oxidase overproduction and alpha-acetolactate decarboxylase inactivation. Eighty percent of the carbon flux was found to be rerouted via alpha-acetolactate to the production of diacetyl by preloading the cells with NADH-oxidase before their use as a cell factory.
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Citations
Hugenholtz, J., Kleerebezem, M., Starrenburg, M., Delcour, J., De Vos, W., & Hols, P. (2000). Lactococcus lactis as a cell factory for high-level diacetyl production. Applied and Environmental Microbiology, 66(9), 4112-4114. https://doi.org/10.1128/AEM.66.9.4112-4114.2000 (Original work published 2000)