Use of anti-cholera toxin IgA-secreting hybridoma cells for the elaboration of an antigen-specific IgA-ELISPOT-assay.
(1990) Immunological Investigations : a journal of molecular and cellular immunology — Vol. 19, n° 2, p. 119-132 (1990)
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- Hybridoma anti-cholera toxin (CT) IgA-secreting cells, because of their monoclonal character, were used to establish and optimize an ELISPOT-assay for CT-specific IgA-secreting cells. The use of hybridoma cells easily and quickly allowed the elaboration of a sensitive, specific and reproducible ELISPOT-assay, which was successfully applied to enumerate intestinal lamina propria lymphoid cells secreting anti-CT IgA in mice immunized twice intraintestinally with CT. Monoclonal antibody-secreting hybridoma cells, if available against a given antigen, are suggested as ideal cell suspensions to elaborate new ELISPOT-assays to study antibody responses at the cellular level.
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Solbreux, P. M., & Vaerman, J.-P. (1990). Use of anti-cholera toxin IgA-secreting hybridoma cells for the elaboration of an antigen-specific IgA-ELISPOT-assay. Immunological Investigations : a journal of molecular and cellular immunology, 19(2), 119-132. https://doi.org/10.3109/08820139009042032 (Original work published 1990)