Actin and creatine in growing soleus and EDL muscle of mouse
(1992) XXth Europ Conf Muscle Contr Cell Motil — Location: Oxford, UK, (23.September.1991)
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- Authors
Maréchal, Georges 
- Abstract
- In soleus and extensor digitorum longus (EDL) muscles of white mice (NMRI) aged 15-100 days, total creatine (Ct = creatine + phosphocreatine) was measured in the supernatant. Actin was separated by electrophoresis on slab gels, stained with Coomassie Blue. Images of the gels were digitized and compared with standard zones of actin. At all ages EDL muscles have higher concentrations of actin and Ct than soleus muscles. When pooled over all ages and muscles (n=30), actin is a linear function of Ct (in µmole/ g muscle: [actin] = 0.055 (± 0.004)*(Ct - 3.8 (± 0.6)). The data suggest existence of two pools of creatine. One pool located in sarcomeres is functionally dependent on actin concentration, keeping a constant ratio of 18 molecules of Ct for every molecule of actin during growth and differentiation into fast or slow muscle. The second pool, presumably located in sarcoplasm remains at a constant concentration of 3.8 µmole per g and may act as a shuttle of ⁓P between the first pool and glycolytic/oxidative metabolic pathways.
Citations
Maréchal, G., & Dillies, P. (1992). Actin and creatine in growing soleus and EDL muscle of mouse. Journal of Muscle Research and Cell Motility, 13(2), 243. https://hdl.handle.net/2078.5/214609 (Original work published 1992)