Investigation of the role of peptones in animal cell culture : case study of a commercial soy peptone in a CHO cell serum-free medium
(2011)
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- (en) Peptones are protein hydrolysates that are widely used as supplement for animal cell cultivation in serum-free media, aiming at increasing process performance (i.e. cell growth and/or protein production). However, their use remains largely empirical and few is understood about the putative mechanisms related to their effects. We first confirmed that the addition of a commercial soy peptone during the batch cultivation of CHO-320 cells in a serum-free medium increased significantly the recombinant g-IFN (g-interferon) production, compared to the cultivation in its absence. For its characterization, the peptone was fractionated by preparative chromatographic methods (ion exchange, gel filtration), generating fractions displaying either beneficial or detrimental effects on g-IFN production by CHO-320 cells. Although some authors hypothesized that peptones could contain a few active compounds activating specific transduction pathways, we were unable to characterize such compounds. However, a fraction improving recombinant g-IFN production but less complex than the whole peptone was obtained. Furthermore, it was observed that the detrimental fractions also exhibit antioxidant activity, which is surprising as it is generally admitted that antioxidants are beneficial in animal cell culture. To further investigate the functional role of peptones on CHO cells, we tried to determine which main cellular functions could be affected by the peptone presence. To this end, effects of the soy peptone on the steps of g-IFN production and secretion were analysed. Although some cellular functions were unaffected, the soy peptone enhanced global protein translation, as it was observed that cellular protein content was increased, and improved the secretion step of g-IFN by CHO-320 cells.
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Michiels, J.-F. (2011). Investigation of the role of peptones in animal cell culture : case study of a commercial soy peptone in a CHO cell serum-free medium. https://hdl.handle.net/2078.5/154932