IL-22 is produced by γC-independent CD25+ CCR6+ innate murine spleen cells upon inflammatory stimuli and contributes to LPS-induced lethality.

Dumoutier, Laure;de Heusch, Magali;Orabona, Ciriana;Satoh-Takayama, Naoko;Renauld, Jean-Christophe;et.al.
(2011) European journal of immunology — Vol. 41, n° 4, p. 1075-1085 (2011)

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Abstract
IL-22 is a Th17 cytokine that plays a key role in immune responses against extracellular bacteria. In mucosal lymphoid tissues, IL-22 production is mainly due to an IL-23-responsive NK-like cell subset that shares some markers with lymphoid tissue inducer (LTi) cells. Here, we identified a new spleen cell population responsible for IL-22 production upon either in vitro stimulation by anti-CD3 antibodies or in vivo stimulation by lipopolysaccharide (LPS) via IL-2- and an IL-23-dependent mechanisms, respectively. These cells represent 1% of spleen cells from recombination activating gene (Rag2)-deficient mice, and correspond to a discrete innate lymphoid cell population expressing CD25, CCR6 and IL-7R. This population comprises 60-70% CD4(+) cells, which produce IL-22, and are still present in common γ chain-deficient mice; the CD4(-) subset coexpresses IL-22 and IL-17, and is common γ chain-dependent. The importance of IL-22 production for the LPS-triggered response is highlighted by the fact that IL-22-deficient mice are more resistant to LPS-induced mortality.
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Dumoutier, L., de Heusch, M., Orabona, C., Satoh-Takayama, N., Eberl, G., Sirard, J.-C., Di Santo, J. P., & Renauld, J.-C. (2011). IL-22 is produced by γC-independent CD25+ CCR6+ innate murine spleen cells upon inflammatory stimuli and contributes to LPS-induced lethality. European journal of immunology, 41(4), 1075-1085. https://doi.org/10.1002/eji.201040878 (Original work published 2011)