Identification of In Vivo Protein Networks Using miniTurbo Proximity Labeling in Bacteria.

Tesseur, Coralie;Remy, Ophélie;Laloux, Géraldine;Santin, Yoann
(2025) Proximity-Dependent Protein Biotinylation : Methods and Protocols — ISBN: [978-1-0716-4694-6], p. 71-80, published

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Abstract
Protein-protein interactions play a crucial role in many bacterial cell processes. miniTurbo-based proximity labeling has emerged as a powerful tool for the identification of in vivo protein networks in bacteria. In this chapter, we outline a standardized protocol for implementing miniTurbo-dependent proximity labeling in bacterial models. This approach enables the unbiased identification of interacting and proximal proteins under native conditions, providing valuable insights into bacterial cell physiology.
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Tesseur, C., Remy, O., Laloux, G., & Santin, Y. (2025). Identification of In Vivo Protein Networks Using miniTurbo Proximity Labeling in Bacteria. In Petra Van Damme (ed.) (ed.), Proximity-Dependent Protein Biotinylation : Methods and Protocols (p. p. 71-80). Humana. https://doi.org/10.1007/978-1-0716-4694-6_5