Densitometric HPTLC quantification of 2-azaanthraquinone isolated from Mitracarpus scaber and antimicrobial activity against Dermatophilus congolensis
(2005) Journal of Planar Chromatography - Modern TLC — Vol. 18, n° 105, p. 377-379 (2005)
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- Abstract
- 2-Azaanthraquinone (benzo[g]isoquinoline) isolated from Mitracarpus scaber has been found to have in-vitro antimicrobial activity against Dermatophilus congolensis (MIC 7.5 mu g mL(-1)), the causative agent of bovine dermatophilosis. To quantify this compound in Mitracarpus scaber, a new, simple and rapid high-performance thin-layer chromatographic (HPTLC) method was developed and validated for selectivity, recovery, and repeatability. Compounds were separated on silica gel 60F(254) plates with toluene-ethyl acetate-methanol, 80 + 18 + 2, as mobile phase. Detection was performed by densitometric scanning at lambda = 310 nm and calibration plots showed the response was linearly dependent on concentration in the range 10-100 mu g mL(-1), with good values of R-2. The method was repeatable and precise with relative standard deviations between 0.98 and 1.59% intra-day and between 3.41 and 5.56% inter-day for concentrations from 10 to 100 mu g mL(-1). Limits of detection and quantification were 3 and 6 mu g mL(-1), respectively (corresponding to 15 and 30 ng, respectively, on the plate). Concentrations of azaanthraquinone were found to be 0.009% in Mitracarpus scaber aerial parts, 0.057% in the alcoholic extract, and 1.95% in the alkaloid extract. Azaanthraquinone may explain the antimicrobial activity of this last extract and part of the effect of the alcoholic extract.
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Gbaguidi, F., Muccioli, G., Accrombessi, G. C., Moudachirou, M., & Quetin-Leclercq, J. (2005). Densitometric HPTLC quantification of 2-azaanthraquinone isolated from Mitracarpus scaber and antimicrobial activity against Dermatophilus congolensis. Journal of Planar Chromatography - Modern TLC, 18(105), 377-379. https://doi.org/10.1556/JPC.18.2005.5.8 (Original work published 2005)