Evaluation of a DNA microarray for rapid detection of the most prevalent extended-spectrum β-lactamases, plasmid-mediated cephalosporinases and carbapenemases in Enterobacteriaceae, Pseudomonas and Acinetobacter.

Bogaerts, Pierre;Cuzon, Gaelle;Evrard, Stéphanie;Hoebeke, Martin;Glupczynski, Gerald;et.al.
(2016) International Journal of Antimicrobial Agents — Vol. 48, n° 2, p. 189-193 (2016)

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Authors
  • Bogaerts, PierreUCLouvain
    Author
  • Cuzon, Gaelle
    Author
  • Evrard, StéphanieUCLouvain
    Author
  • Hoebeke, MartinUCLouvain
    Author
  • Glupczynski, GeraldUCLouvain
    Author
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Abstract
The dissemination of Gram-negative bacteria (GNB) producing extended-spectrum β-lactamases (ESBLs), plasmid-encoded cephalosporinases (pAmpCs) and carbapenemases is a matter of great clinical concern. In this study, we evaluated a new low-density DNA array 'Check-MDR CT103 XL' (Check-Points, Wageningen, The Netherlands) that identifies the most clinically relevant β-lactamase genes of ESBLs (blaTEM, blaSHV, blaCTX-M, blaBEL, blaPER, blaGES and blaVEB), pAmpCs (blaCMY-2-like, blaDHA, blaFOX, blaACC-1, blaACT/MIR and blaCMY-1-like/MOX) and carbapenemases (blaKPC, blaOXA-48, blaVIM, blaIMP, blaNDM, blaGIM, blaSPM and blaOXA-23, -24 and -58) in cultured bacteria. In total, 223 GNB isolates with well-characterised resistance mechanisms to β-lactams were analysed. A specificity and sensitivity of 100% were recorded for most bla genes, with a slightly lower signal observed for blaIMP. The Check-MDR CT103 XL array proved highly accurate for the identification of epidemiologically relevant ESBL, pAmpC and carbapenemase genes harboured in Enterobacteriaceae, Pseudomonas and Acinetobacter spp. The Check-MDR CT103 XL assay is a significant improvement compared with Check-MDR CT103 and it highlights the ability of this array to evolve rapidly to adjust to the current needs for the detection of resistance mechanisms to β-lactam agents.
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Bogaerts, P., Cuzon, G., Evrard, S., Hoebeke, M., Naas, T., & Glupczynski, G. (2016). Evaluation of a DNA microarray for rapid detection of the most prevalent extended-spectrum β-lactamases, plasmid-mediated cephalosporinases and carbapenemases in Enterobacteriaceae, Pseudomonas and Acinetobacter. International Journal of Antimicrobial Agents, 48(2), 189-193. https://doi.org/10.1016/j.ijantimicag.2016.05.006 (Original work published 2016)