Endothelial type nitric oxide synthase in skeletal muscle fibers : mitochondrial relationships.
(1995) Biochemical and Biophysical Research Communications — Vol. 211, n° 2, p. 375-381 (1995)
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- Abstract
- Immunohistochemistry shows that skeletal muscle fibers express endothelial-type (ec)-NOS in a heterogeneous pattern distinct from that of neuronal (nc)-NOS. Analysis of adjacent serial sections reveals: 1) a strong correlation of ec-NOS expression to mitochondrial content (visualized histochemically by succinate dehydrogenase); 2) lack of correlation of ec-NOS to fiber ATPase type (in contrast to nc-NOS); and 3) constitutive co-expression of ec- and nc-NOS in some fibers. Preparations of mitochondria from diaphragm exhibited calcium-dependent NOS activity, which functioned to inhibit in vitro oxygen consumption. The results establish that normal skeletal muscle cells can express two constitutive isoforms of NOS (nc- and ec-) and suggest a functional role for ec-NOS in oxidative, mitochondria-rich fibers.
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Kobzik, L., Stringer, B., Balligand, J.-L., Reid, M. B., & Stamler, J. S. (1995). Endothelial type nitric oxide synthase in skeletal muscle fibers : mitochondrial relationships. Biochemical and Biophysical Research Communications, 211(2), 375-381. https://doi.org/10.1006/bbrc.1995.1824 (Original work published 1995)