How plasma membrane properties contribute to red blood cell vesiculation
(2022)
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THESIS-Anne-SophieCloos.pdf
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Details
- Authors
- Supervisors
- Tyteca, Donatienne
- Abstract
- The loss of plasma membrane from red blood cells (RBCs) through the budding and release of extracellular vesicles (EVs) affects their shape, deformability and functionality. Although oxidative stress appears as the main triggering factor for RBC vesiculation, the role of plasma membrane properties is largely overlooked. We here addressed if plasma membrane biophysical properties as well as lipid transversal and lateral distribution could contribute to the RBC vesiculation process upon RBC storage and in hereditary spherocytosis, a membrane fragility disease. Results indicate that, in contrast to spherocytosis, the release of EVs during hypothermic storage in blood tubes and in RBC concentrates is accompanied by the successive impairment of RBC membrane rigidity, lateral heterogeneity in lipid domains and transversal asymmetry. Our data open the way to use EVs or lipid domains for quality control in transfusion centers and to target domains to minimize vesiculation in RBC concentrates.
- Affiliations
Citations
Cloos, A.-S. (2022). How plasma membrane properties contribute to red blood cell vesiculation.