Evaluation of chromogenic and selective media for the detection of Pseudomonas aeruginosa and Staphylococcus aureus in respiratory samples from cystic fibrosis patients

André, Emmanuel;Lebecque, Patrick;SIMON, Anne;Huang, Te-Din
(2010) 20th European Congress of Clinical Microbiology and Infectious Diseases — Location: Vienne, Autriche (10.April.2010)

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Authors
  • André, EmmanuelUCLouvain
    Author
  • Lebecque, PatrickUCLouvain
    Author
  • SIMON, AnneUCLouvain
    Author
  • Huang, Te-DinUCLouvain
    Author
Abstract
Objectives : Early detection of Pseudomonas aeruginosa (PA) and Staphylococcus aureus (SA) infection in cystic fibrosis (CF) patients remains a challenge for the microbiology lab. The aim of this study was to compare commercial selective media to standard culture media for the recovery and the presumptive identification of PA and of SA directly from sputum collected in these patients. Methods: 67 sputa from 67 ambulatory CF patients were cultured onto 6 selective media including MacConkey agar (MC; home-made), Pseudomonas Isolation Agar (PABD; Becton-Dickinson fig 1), ChromID PA agar (PABM; bioMérieux – fig 2), a colistin aztreonam blood agar plate (CAP, Oxoid), CHROMagar SA medium (SABD; BD – fig 3) and ChromID SA agar (SABM; bioMérieux – fig 4). After up to 7 days of incubation at 35°C, colonies suggestive of PA or SA according to the plates reading guidelines provided by the manufacturers were selected for phenotypic identification. Isolates showing atypical testing results were further submitted for molecular identification by 16S rRNA sequencing. Conclusions : The use of specific selective media could increase the recovery of PA and SA isolates in CF respiratory samples and could lead to an earlier management and antimicrobial therapy for these colonized or infected patients. Nevertheless, the relatively low specificity of these media doesn’t exempt the microbiologist from usual confirmative test.

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André, E., Lebecque, P., SIMON, A., & Huang, T.-D. (2010). Evaluation of chromogenic and selective media for the detection of Pseudomonas aeruginosa and Staphylococcus aureus in respiratory samples from cystic fibrosis patients. 20th European Congress of Clinical Microbiology and Infectious Diseases, Vienne, Autriche. https://hdl.handle.net/2078.5/66949