[Regeneration of Striated Muscles From Cultured Isolated Myoblasts]
(1984)
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- Authors
Maréchal, Georges 
- Abstract
- (en) The triceps surae of a female Wistar rat (aged 4 to 5 weeks) is minced and grafted orthotopically. In a few weeks a new muscle regenerates, vascularized, innervated and functional. One month after the procedure, his maximum isometric strength was 0.4 g. If at the time of surgery, the muscle is frozen with liquid nitrogen, thawed and heated for 10 minutes at 65 °C, just before the transplantation, regeneration of muscle fibers is completely removed. A conjunctive-fatty tissue is then formed, devoid of any muscle fiber. We seeded the frozen the right triceps surae and baked with one to two million cells, including about 10% myoblasts (estimate based on the proportion of myotubes formed in the culture). These cells were isolated one wek before transplant from the left triceps of the same animal and grown in a proliferative medium containing 20% fetal calf serum. The regenerate is then contractile, though weakly. One month after the procedure, the maximum isometric force was 0.4 g in one case, 0.45 g in a second case and 2 g in a third case.
- Affiliations
Citations
Ghins, E., & Maréchal, G. (1984). [Regeneration of Striated Muscles From Cultured Isolated Myoblasts]. Journal de Physiologie, 79(1), A25-A25. (Original work published 1984)