Ly-6A/E induction by interleukin-6 and interleukin-9 in T cells
(1999) European Cytokine Network — Vol. 10, n° 1, p. 49-56 (1999)
Files
83974.pdf
Restricted Access - Adobe PDF
- 3.86 MB
Details
- Authors
- Abstract
- Interleukin-6 (IL-6) is active in the early steps of T cell activation and confers IL-2 responsiveness. In this work, we used EL4 T lymphoma to identify IL-6 target genes in T cells. By differential screening of a cDNA library, we found that IL-6 induced the expression of Ly-6A/E, a GPI-anchored cell surface protein reported to be a regulator of T cell activation. In addition to IL-6, IL-9 and IFN-gamma induced Ly-6A/E expression in EL4 and BW5147 cells. We showed that both IL-6 and IL-9 mediated the transcriptional activation of Ly-6A/E through a GAS element in the Ly-6A/E promoter, which was able to bind STAT1 and STAT3, transcription factors activated by these cytokines. IL-6 had a similar effect in freshly isolated normal T cells, and dramatically increased their proliferation upon Ly-6A/E stimulation. Taken together, our data suggest that Ly-6A/E induction takes part in the T cell activation program initiated by IL-6.
- Affiliations
Citations
Demoulin, J. B., Maisin, D., & Renauld, J.-C. (1999). Ly-6A/E induction by interleukin-6 and interleukin-9 in T cells. European Cytokine Network, 10(1), 49-56. https://hdl.handle.net/2078.5/153992 (Original work published 1999)