Cytotoxic responses to reference moist smokeless tobacco extracts in a three dimensional oral cell culture system
(2010) 49th Annual Meeting of the Society of Toxicology — Location: Salt Lake city, Utah, USA
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- Abstract
- The use of smokeless tobacco products at the same oral site may result in an acute injury characterized by ulceration and inflammation that is reversible upon elimination of same site use. However, little research has been conducted on in vitro models that mimic the human oral cavity response to smokeless tobacco exposure. In the current study we used the 3D oral mucosal system, EpiOralTM, to investigate the responses of oral mucosal tissue to highly concentrated extracts of reference moist smokeless tobacco (MST) that reflect exposure at the site of MST use in the human oral cavity. The responses observed following 16hr of MST exposure include loss of cell viability (detected by MTT), induction of inflammatory mediator gene expression (detected by RT-PCR) and protein release (detected by cytometric bead assay and ELISA) and ultrastructural changes as measured by scanning electron and light microscopy. In addition, 2D differential gel electrophoresis was conducted on the culture supernatants and identified 31 unique proteins that were differentially modulated by MST extracts. The released proteins were analyzed for their associated functional class and revealed proteins involved in proteolysis, oxidative stress, calcium binding and cytoskeletal function. In conclusion, responses observed in the EpiOralTM model system are similar to those reported for the human oral mucosa during MST use. Thus, the EpiOralTM system is a promising model for evaluating tissue responses to smokeless tobacco extracts.
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Langston, B., Edminston, J., Scian, M., Lombard, C., Roberts, O., Baliga, V., Fisher, M., Fariss, M., & Flora, J. (2010). Cytotoxic responses to reference moist smokeless tobacco extracts in a three dimensional oral cell culture system. Toxicological Sciences, 114(1 supplement), 160. https://hdl.handle.net/2078.5/83103 (Original work published 2010)