Cryopreservation of entrapped monoxenically produced spores of an arbuscular mycorrhizal fungus
(2000) New Phytologist — Vol. 148, n° 1, p. 169-176 (2000)
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- A study was conducted to quantify the ability of entrapped, monoxenically produced spores of an arbuscular mycorrhizal fungus to germinate and reproduce the fungal life cycle after crpopreservation. No germination was obtained after incubation of entrapped spores in glycerol and mannitol and subsequent cryopreservation at -70 degreesC, regardless of the concentration of cryoprotectants and duration of incubation. Incubation for 1 d in 0.5 M sucrose, and for 1 and 2 d in 0.5 M trehalose, led to spore germination after cryopreservation at - 70 degreesC. Lower cryopreservation temperatures were tested with entrapped spores incubated for 1 d in 0.5 M trehalose. The highest germination rate, estimated by the percentage of potentially infective beads (%PIB), was obtained at -100 degreesC. A %PIB of 95% (water agar medium) to 100% (Strullu-Romand medium) was obtained at this temperature. Thereafter, %PIB rapidly decreased at -140 and -180 degreesC. Heavy sporulation and high internal root colonization were obtained after re-association of the entrapped spores, incubated for 1 d in 0.5 M trehalose and subsequently cryopreserved at -100 degreesC, with transformed carrot roots. This demonstrates the ability of entrapped spores to reproduce the fungal life cycle following cold treatment.
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Declerck, S., & Angelo-Van Coppenolle, M. (2000). Cryopreservation of entrapped monoxenically produced spores of an arbuscular mycorrhizal fungus. New Phytologist, 148(1), 169-176. https://doi.org/10.1046/j.1469-8137.2000.00740.x (Original work published 2000)