The NeuAc(alpha-2,6)Gal/GalNAc-binding lectin from elderberry (Sambucus nigra) bark, a type-2 ribosome-inactivating protein with an unusual specificity and structure
(1996) European Journal of Biochemistry — Vol. 235, n° 1-2, p. 128-137 (1996)
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- Abstract
- The cDNA encoding the NeuAc(alpha-2,6)Gal/GalAc binding lectin from elderberry (Sambucus nigra) bark (SNAI) was isolated from a cDNA library constructed with mRNA from the bark. Sequence analysis of this lectin cDNA revealed a striking similarity to the previously sequenced type-2 ribosome-inactivating proteins from Ricinus communis and Abrus precatorius. Molecular modelling of SNAI further indicated that its structure closely resembles that of ricin. Since SNAI strongly inhibits cell-free protein synthesis in a rabbit reticulocyte lysate it presumably is a type-2 ribosome-inactivating protein. However, SNAI differs from all previously described type-2 ribosome-inactivating proteins by its specificity towards NeuAc(alpha-2,6)Gal/GalNAc and its unusual molecular structure.
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VanDamme, E., Barre, A., Rouge, P., VanLeuven, F., & Peumans, W. (1996). The NeuAc(alpha-2,6)Gal/GalNAc-binding lectin from elderberry (Sambucus nigra) bark, a type-2 ribosome-inactivating protein with an unusual specificity and structure. European Journal of Biochemistry, 235(1-2), 128-137. https://doi.org/10.1111/j.1432-1033.1996.00128.x (Original work published 1996)