Induction of apoptosis in human promyelocytic leukemia cells by a natural trachylobane diterpene
(2005) Anticancer Research : international journal of cancer research and treatment — Vol. 25, n° 1A, p. 363-368 (2005)
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- BACKGROUND: Trachylobane diterpenes are secondary metabolites, quite rare in nature, and their bioactivities are poorly understood. Recently, we have described the cytotoxic activity of ent-trachyloban-3beta-ol isolated from the leaves of Croton zambesicus, a plant used in African folk medicine. MATERIALS AND METHODS: Cell viability on several cell lines, cell morphology, DNA laddering, annexin Vand caspase-3 activation experiments were undertaken in order to analyse the cytotoxicty of trachylobane diterpene and to determine if this compound is able to induce apoptosis. RESULTS: ent-Trachyloban-3beta-ol exerts a dose-dependent cytotoxic effect, which varies between cell lines. Induction of apoptosis in HL-60 cells could be detected at a concentration of 50 microM after 24-h treatment. CONCLUSION: We show here, for the first time, that a trachylobane diterpene is able to induce apoptosis in human promyelocytic leukemia cells via caspase-3 activation in a concentration-dependent manner.
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Blockx, S., Gerkens, P., Peulen, O., Jolois, O., Mingeot-Leclercq, M.-P., De Pauw-Gillet, M.-C., & Quetin-Leclercq, J. (2005). Induction of apoptosis in human promyelocytic leukemia cells by a natural trachylobane diterpene. Anticancer Research : international journal of cancer research and treatment, 25(1A), 363-368. https://hdl.handle.net/2078.5/99192 (Original work published 2005)