A practical molecular clip for immobilization of receptors and biomolecules on devices' surface: synthesis, grafting protocol and analytical assay.

Devouge, Sabrina;Salvagnini, Claudio;Marchand-Brynaert, Jacqueline
(2005) Bioorganic & Medicinal Chemistry Letters : the tetrahedron journal for research at the interface of chemistry and biology — Vol. 15, n° 13, p. 3252-3256 (2005)

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Authors
  • Devouge, SabrinaUCLouvain
    Author
  • Salvagnini, ClaudioUCLouvain
    Author
  • Marchand-Brynaert, JacquelineUCLouvain
    Author
Abstract
Deposition of O-succinimidyl 4-(p-azido-phenyl)butanoate (4) onto inorganic device (FTIR-ATR crystal) or polymer material (filtration membrane) followed by irradiation at 254nm led to surface functionalisation with NHS esters. Further reaction with biomolecules allowed their covalent grafting. The reactivity of the photoactivated surfaces was assayed by two methods: (i) the coupling with 3,5-bis(trifluoromethyl)benzylamine (7) and subsequent XPS analysis; (ii) the coupling with 4,5-bis-tritiated lysine (10) and subsequent LSC measurement of the radioactivity.
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Devouge, S., Salvagnini, C., & Marchand-Brynaert, J. (2005). A practical molecular clip for immobilization of receptors and biomolecules on devices’ surface: synthesis, grafting protocol and analytical assay. Bioorganic & Medicinal Chemistry Letters : the tetrahedron journal for research at the interface of chemistry and biology, 15(13), 3252-3256. https://doi.org/10.1016/j.bmcl.2005.04.071 (Original work published 2005)