Immunological modulation following bone marrow-derived mesenchymal stromal cells and Th17 lymphocyte co-cultures.

Najar, Mehdi;Fayyad-Kazan, Hussein;Faour, Wissam H;Merimi, Makram;Fahmi, Hassan;et.al.
(2019) Inflammation Research — Vol. 68, n° 3, p. 203-213 (2019)

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Authors
  • Najar, Mehdi
    Author
  • Fayyad-Kazan, Hussein
    Author
  • Faour, Wissam H
    Author
  • Merimi, Makram
    Author
  • Sokal, Etienneorcid-logoUCLouvain
    Author
  • Lombard, CatherineUCLouvain
    Author
  • Fahmi, Hassan
    Author
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Abstract
OBJECTIVE AND DESIGN: The objective of the study is to uncover the influence of human bone marrow-derived mesenchymal stem cells (BM-MSCs) on the generation of Th17 lymphocytes in co-cultures of both BM-MSCs and T cells. MATERIALS AND METHODS: BM-MSCs, characterized according to the international society for cellular therapy (ISCT) criteria, were co-cultured with T cells isolated from peripheral blood. The expression levels of IL-17 receptor, RORγt and IL-23 receptor were evaluated using flow cytometry. The levels of cytokines involved in Th17 immunomodulation were measured using multiplex assay. TREATMENT: Inflammatory primed and non-primed BM-MSCs were co-cultured with either activated or non-activated T cells either at (1/80) and (1/5) ratio respectively. RESULTS: MSC/T-cell ratio and inflammation significantly influenced the effect of BM-MSCs on the generation of Th17 lymphocytes. Cocultures of either primed or non-primed BM-MSCs with activated T cells significantly induced IL-17A-expressing lymphocytes. Interestingly, the expression of the transcription factor RORγt was significantly increased when compared to levels in activated T cells. Finally, both cell ratio and priming of BM-MSCs with cytokines substantially influenced the cytokine profile of BM-MSCs and T cells. CONCLUSION: Our findings suggest that BM-MSCs significantly modulate the Th17 lymphocyte pathway in a complex manner.
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Najar, M., Fayyad-Kazan, H., Faour, W. H., Merimi, M., Sokal, E., Lombard, C., & Fahmi, H. (2019). Immunological modulation following bone marrow-derived mesenchymal stromal cells and Th17 lymphocyte co-cultures. Inflammation Research, 68(3), 203-213. https://doi.org/10.1007/s00011-018-1205-0 (Original work published 2019)