Isolation, pure culture and characterization of Serratia symbiotica sp. nov., the R-type of secondary endosymbiont of the black bean aphid Aphis fabae

Sabri, Ahmed;Leroy, Pascal;Haubruge, Eric;Hance, Thierry;Thonart, Philippe;et.al.
(2011) International Journal of Systematic and Evolutionary Microbiology — Vol. 61, n° 10, p. 2081-2088 (2011)

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Authors
  • Sabri, AhmedUniversity of Liege
    Author
  • Leroy, PascalAgro-Bio Tech Gembloux, Belgium
    Author
  • Haubruge, EricAgro-Bio Tech Gembloux, Belgium
    Author
  • Hance, ThierryUCLouvain
    Author
  • Frere, IsabelleUCLouvain
    Author
  • Thonart, PhilippeUniversity of Liege
    Author
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Abstract
An intracellular symbiotic bacterium was isolated from the flora of a natural clone of the black bean aphid Aphis fabae. The strain was able to grow freely in aerobic conditions on a rich medium containing 1% of each of the following substrates: glucose, yeast extract and casein peptone. Pure culture was achieved through the use of solid-phase culture on the same medium and the strain was designated CWBI-2.3T. 16S rRNA gene sequence analysis revealed that strain CWBI-2.3T was a member of the class Gammaproteobacteria, having high sequence similarity (.99 %) with ‘Candidatus Serratia symbiotica’, the R-type of secondary endosymbiont that is found in several aphid species. As strain CWBI-2.3T (5LMG 25624T5DSM 23270T) was the first R-type symbiont to be isolated and characterized, it was designated as the type strain of Serratia symbiotica sp. nov.
Affiliations
  • University of LiegeCWBI
  • Agro-Bio Tech Gembloux, BelgiumDepartment of Functional and Evolutionary Entomology
  • Agro-Bio Tech Gembloux, BelgiumDepartment of Functional and Evolutionary Entomology
  • Institution iconUCLouvainSST/ELI/ELIB - Biodiversity

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Sabri, A., Leroy, P., Haubruge, E., Hance, T., Frere, I., Destain, J., & Thonart, P. (2011). Isolation, pure culture and characterization of Serratia symbiotica sp. nov., the R-type of secondary endosymbiont of the black bean aphid Aphis fabae. International Journal of Systematic and Evolutionary Microbiology, 61(10), 2081-2088. https://hdl.handle.net/2078.5/39246 (Original work published 2011)