Regulation of the Expression of the H+-atpase Genes Pma1 and Pma2 During Growth and Effects of Octanoic-acid in Saccharomyces-cerevisiae
(1994) BBA - Gene Structure and Expression — Vol. 1217, n° 1, p. 74-80 (1994)
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- A peak of plasma membrane H+-ATPase activity during exponential growth is correlated with the expression of the PMA1 gene as monitored by measurements of the beta-galactosidase activity from a PMA1-lacZ fusion. This peak of activity is also correlated to the content of the H+-ATPase protein in yeast plasma membrane as shown by quantitative immunodetection. The PMA(2)-lacZ fusion assay indicates that the expression of the PMA2 gene is activated somewhat later during exponential phase but under all circumstances its activity remains at least 500-fold lower than that of the PMA1-lacZ fusion. A slight but significant stimulation of ATPase activity by low concentrations of octanoic acid coincides with a decrease in the PMA1 gene expression. It is concluded that octanoic acid stimulates de PMA1 ATPase activity by posttranslational mechanisms.
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Viegas, CA., Supply, P., Capieaux, E., Vandyck, L., Goffeau, A., & Sacorreia, I. (1994). Regulation of the Expression of the H+-atpase Genes Pma1 and Pma2 During Growth and Effects of Octanoic-acid in Saccharomyces-cerevisiae. BBA - Gene Structure and Expression, 1217(1), 74-80. https://doi.org/10.1016/0167-4781(94)90126-0 (Original work published 1994)