Latex immunoassay of serum alpha-fetoprotein using polyethylene glycol pretreatment.
(1988) Journal of Immunological Methods — Vol. 109, n° 1, p. 69-74 (1988)
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- Abstract
- A latex particle immunoassay has been developed for the determination of serum alpha-fetoprotein. The assay consists of incubating the serum sample for 30 min at 50 degrees C with latex particles coated with anti-alpha-fetoprotein immunoglobulin, then quantifying the remaining unagglutinated particles with an optical particle counter. The assay is fully automated with a sampling rate of 40/h. The interference from rheumatoid factor is eliminated by precipitation with polyethylene glycol (7% final concentration). The alpha-fetoprotein standard curve, prepared in a human serum matrix, extends from 0.5 to 32 micrograms/l. Because sera are diluted ten-fold, the limit of detection of the assay lies around 5 micrograms/l. Coefficients of variation ranged from 4 to 15% and the recovery of alpha-fetoprotein tested on eight normal sera and four sera containing rheumatoid factor averaged 102.2% (SD = 13.2). The correlation coefficient between latex immunoassay and radioimmunoassay, calculated from the assay of 138 samples was 0.98.
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Passelecq, B., De Bo, M., Huber, C., Gennart, J. P., Bernard, A., & Lauwerys, R. (1988). Latex immunoassay of serum alpha-fetoprotein using polyethylene glycol pretreatment. Journal of Immunological Methods, 109(1), 69-74. https://doi.org/10.1016/0022-1759(88)90443-7 (Original work published 1988)