Quantitative Estimation of Heterocyclic Aromatic-amines By Ion-exchange Chromatography and Electrochemical Detection
(1995) 20th International Symposium on Chromatography — Location: BOURNEMOUTH(England) (19.June.1994)
Files
pdfdocument.pdf
Restricted Access - Adobe PDF
- 363.25 KB
Details
- Authors
- Abstract
- Conditions for the quantitative determination of three heterocyclic aromatic amines (HAAs), produced by heat processing of protein-rich food products, were established for a HPLC-electrochemical detection system. The separation of 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (MeIQ) and 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQ(x)) was achieved on an ion-exchange column with acetonitrile-80 mM phosphate buffer (30:70) mobile phase at pH 5.6. The figures of merit were calculated. Reproducibility gave a relative standard deviation of 1.6-2.4% when measured by peak area. Detection limits (signal-to-noise ratio 3) ranged from 35 pg for MeIQ(x) to 70 pg for MeIQ. The method was applied to the determination of HAAs in a commercial beef extract sample.
- Affiliations
Citations
Vandyck, MMC., Rollmann, B., & De Meester, C. (1995). Quantitative Estimation of Heterocyclic Aromatic-amines By Ion-exchange Chromatography and Electrochemical Detection. Journal of Chromatography A, 697(1-2), 377-382. https://doi.org/10.1016/0021-9673(94)00837-Y (Original work published 1995)