A full-length infecious clone of beet soil-borne virus indicates the dispensability of the RNA-2 for virus survival in planta and symptom expression on Chenopodium quinoa leaves.
(2009) Journal of General Virology — Vol. 90, p. 3051-3056 (2009)
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- Authors
- Abstract
- For a better understanding of the functionality and pathogenicity of beet soil-borne virus (BSBV) full-length cDNA clones have been constructed for the three genomic RNAs. With the aim of asessing their effectivenes and relative conribution to the virus housekeeping functions, transcripts were inoculated on Chenopodium quinoa and Beta macrocarpa leaves using five genome combinations. Both RNAs-1(putative replicase) and -3 (putative movement proteins) proved to be essential for virus replication in planta and symptom production on C. quinoa, whereas RNA-2 (putative coat protein, CP, and a read-through domain, RT) was not . No symptoms were recorded on B.macrocarpa, but vital RNAs were detected. In both host plants, the 19kDa CP was deteced by Western blotting as well as 115kDa protein corresponding to the CP-RT.
- Affiliations
Institut de Biologie Moléculaire des Plantes Laboratoire propre du CNRS conventionné avec l'Université de Strasbourg
Citations
Crutzen, F., Mehrvar, M., Gilmer, D., & Bragard, C. (2009). A full-length infecious clone of beet soil-borne virus indicates the dispensability of the RNA-2 for virus survival in planta and symptom expression on Chenopodium quinoa leaves. Journal of General Virology, 90, 3051-3056. https://doi.org/10.1099/vir.0.014548-0 (Original work published 2009)