Cryogenic contrast-enhanced microCT (cryo-CECT) enables 3D quantitative histopathology of soft biological tissues

Maes, Arne;et.al.
(2022) Belgian Symposium on Tissue Engineering (BSTE) 2022 — Location: Antwerp, belgium (2.December.2022)

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  • Maes, Arneorcid-logoUCLouvain
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  • et. al.
Abstract
Cryo-CECT was first optimized in terms of CESA and freezing rate using bovine muscle tissue (Fig. 1 a-d). We then applied cryo-CECT on murine hearts that underwent transverse aortic constriction (TAC) surgery, a well-established experimental model for pressure overload-induced cardiac hypertrophy. Cryo-CECT allowed to analyze, in an unprecedented manner, the orientation and diameter of the individual muscle fibers in the entire heart (Fig. 1 e), as well as the 3D localization of fibrotic regions within the myocardial layers (Fig. 1 f). With this analysis, we demonstrated the added value and complimentary of cryo-CECT to conventional histopathological tools.
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Maes, A., & et al. (2022). Cryogenic contrast-enhanced microCT (cryo-CECT) enables 3D quantitative histopathology of soft biological tissues. Belgian Symposium on Tissue Engineering (BSTE) 2022, Antwerp, belgium. https://hdl.handle.net/2078.5/102962