Identification and Quantification of a Naturally Presented Peptide As Recognized By Cytotoxic Lymphocytes-t Specific for An Immunogenic Tumor Variant
(1992) International Immunology — Vol. 4, n° 10, p. 1085-1090 (1992)
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- Abstract
- The target antigen recognized by H-2K(d)-restricted cytotoxic T lymphocytes (CTLs) specific for a mutagen-induced antigen on DBA/2-derived tumor P815 was identified as the product of a normal cellular gene encompassing a point mutation. Using synthetic peptides, the epitope recognized by these CTLs was narrowed down to be contained within the undecamer KYQAVTTTLEE, incorporating the point mutation. The allele-specific peptide motif for H-2K(d) molecules allowed us to predict the peptide naturally presented by the tumor cells to be the nonamer KYQAVTTTL. Isolation of the natural tum--specific peptide from P198.3 tumor cells and biochemical comparison with the synthetic nonamer confirmed the prediction. This natural nonapeptide is represented by approximately 1 00 copies per tumor cell.
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Wallny, HJ., Deres, K., Faath, S., Jung, G., Van Pel, A., Boon, T., & Rammensee, HG. (1992). Identification and Quantification of a Naturally Presented Peptide As Recognized By Cytotoxic Lymphocytes-t Specific for An Immunogenic Tumor Variant. International Immunology, 4(10), 1085-1090. https://doi.org/10.1093/intimm/4.10.1085 (Original work published 1992)