New frontiers in atomic force microscopy: analyzing interactions from single-molecules to cells

Mueller, Daniel J.;Krieg, Michael;Alsteens, David;Dufrêne, Yves
(2009) Current Opinion in Biotechnology — Vol. 20, n° 1, p. 4-13 (2009)

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Originally invented for imaging surfaces, atomic force microscopy (AFM) has evolved into a multifunctional molecular toolkit, enabling us to investigate the interactions of biological systems over scales ranging from single-molecules to whole cells. Specific highlights include the nanoscale imaging of the chemical properties of individual cells, the detection and functional analysis of cell surface receptors using single-molecule force spectroscopy and the quantitative measurement of cellular interactions using single-cell force spectroscopy. These advanced force spectroscopy modalities offer new opportunities for understanding the molecular bases of cell adhesion processes, which is a fundamental challenge in current life science and biotech research.
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Mueller, D. J., Krieg, M., Alsteens, D., & Dufrêne, Y. (2009). New frontiers in atomic force microscopy: analyzing interactions from single-molecules to cells. Current Opinion in Biotechnology, 20(1), 4-13. https://doi.org/10.1016/j.copbio.2009.02.005 (Original work published 2009)