Species identification of corynebacteria by cellular fatty acid analysis.
(2006) Diagnostic Microbiology and Infectious Disease — Vol. 54, n° 2, p. 99-104 (2006)
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- Abstract
- We evaluated the usefulness of cellular fatty acid analysis for the identification of corynebacteria. Therefore, 219 well-characterized strains belonging to 21 Corynebacterium species were analyzed with the Sherlock System of MIDI (Newark, DE). Most Corynebacterium species have a qualitative different fatty acid profile. Corynebacterium coyleae (subgroup 1), Corynebacterium riegelii, Corynebacterium simulans, and Corynebacterium imitans differ only quantitatively. Corynebacterium afermentans afermentans and C. coyleae (subgroup 2) have both a similar qualitative and quantitative profile. The commercially available database (CLIN 40, MIDI) identified only one third of the 219 strains correctly at the species level. We created a new database with these 219 strains. This new database was tested with 34 clinical isolates and could identify 29 strains correctly. Strains that remained unidentified were 2 Corynebacterium aurimucosum (not included in our database), 1 C. afermentans afermentans, and 2 Corynebacterium pseudodiphtheriticum. Cellular fatty acid analysis with a self-created database can be used for the identification and differentiation of corynebacteria.
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Van den Velde, S., Lagrou, K., Desmet, K., Wauters, G., & Verhaegen, J. (2006). Species identification of corynebacteria by cellular fatty acid analysis. Diagnostic Microbiology and Infectious Disease, 54(2), 99-104. https://doi.org/10.1016/j.diagmicrobio.2005.08.019 (Original work published 2006)