The iNanoBIT project: integration of Nano - and Biotechnology for Beta-cell and Islet Transplantation
(2019) 15th Congress of the International Xenotransplantation Association (IXA 2019) — Location: Munich, Germany (10.October.2019)
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- Authors
Gianello, Pierre 
- Abstract
- Preclinical trials with porcine pancreatic islets have started and in recent years major progress has been made, particularly in the development of novel immunosuppressive regimens and genetically multi-modified donor pigs with an excellent potential to become a clinically relevant option for the treatment of type-1 diabetes (T1D). Ethical and regulatory frameworks for this new branch of medicine are currently being developed, including safety requirements. High resolution imaging of the function and faith of transplanted porcine xenoislets and beta-cells in large animals and patients for testing ATMPs is a currently unmet need for preclinical/clinical testing. The iNanoBIT EU H2020 project is aimed to apply nanotechnologies for imaging porcine pancreatic islet cellular transplants and induced pluripotent stem cell-derived beta-cells and subsequent regenerative processes in vivo in a porcine model. The project is developing novel highly sensitive nanotechnology-based imaging approaches allowing for monitoring of survival, engraftment, proliferation, function and whole body distribution of the cellular transplants in a preclinical porcine model with excellent translational potential to humans. We develop and validate the application of SPECT and optoacoustic imaging technologies in preclinical transgenic humanized type-1 diabetic pig model to observe transplanted porcine xenoislets and in vitro differentiated human beta-cells. The consortium of 5 SME and 3 Academic partners is progressing in generating new transgenic reporter pigs and human induced pluripotent cell lines for optoacoustic imaging and testing them in transplantable bioartificial islet devices. Novel nanomolecules have been generated and being tested for nuclear imaging of islets and beta-cells using a new, high-resolution SPECT imaging device for large animals. Overall, the combined multidisciplinary expertise of the project partners allows progress towards creating much needed technological tool-boxes for the xenotransplantation and ATMP field, and thus reinforce the European healthcare supply chain for regenerative medicinal products.
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Citations
Dinnyes, A., Bartenstein, P., Bouaoun, R., Burton, N., Cyran, C., Gianello, P., Kemter, E., Kobolak, J., Nemeth, G., Nicotra, F., Prepost, E., Qiu, Y., Russo, L., Wirth, A., Wolf, E., & Ziegler, S. (2019). The iNanoBIT project: integration of Nano - and Biotechnology for Beta-cell and Islet Transplantation. Xenotransplantation, 26(5), e12553 (83). https://hdl.handle.net/2078.5/97160 (Original work published 2019)