Comparison between the orthorhombic and tetragonal forms of the heptamer sequence d[GCG(xT)GCG]/d(CGCACGC)
(2010) Acta Crystallographica Section F: Structural Biology and Crystallization Communications — Vol. 66, n° 9, p. 1028-1031 (2010)
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- Abstract
- Cyclohexene nucleic acid (CeNA) building blocks can be introduced into natural DNA sequences without a large conformational influence because of the ability of the six-membered sugar ring to mimic both the C2′-endo and C3′-endo conformations of the naturally occurring ribofuranose sugar ring. The non-self-complementary DNA sequence d[GCG(xT)GCG]/d(CGCACGC) with one incorporated CeNA (xT) moiety crystallizes in two forms: orthorhombic and tetragonal. The tetragonal form, which diffracts to 3 Å resolution, is a kinetically stable polymorph of the orthorhombic form [Robeyns et al. (2010), Artificial DNA, 1, 1-7], which diffracts to 1.17 Å resolution and is the thermodynamically stable form of the CeNA-incorporated duplex. Here, the two structures are compared, with special emphasis on the differences in crystal packing and the irreversible conversion of the kinetic form into the high-resolution diffracting thermodynamic form. © 2010 International Union of Crystallography All rights reserved.
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Robeyns, K., Herdewijn, P., & Van Meervelt, L. (2010). Comparison between the orthorhombic and tetragonal forms of the heptamer sequence d[GCG(xT)GCG]/d(CGCACGC). Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 66(9), 1028-1031. https://doi.org/10.1107/S1744309110031696 (Original work published 2010)