A New Production Method for Glucose-oxidase

Debaetselier, A.;Dohet, P.;De Beukelaer, Michèle;Hathi, V.;Vasavada, A.;et.al.
(1992) INTERNATIONAL CONF ON THE INDUSTRIAL APPLICATIONS OF NATURAL, MODIFIED AND ARTIFICIAL ENZYMES — Location: PISA(Italy) (23.September.1990)

Files

pdfdocument.pdf
  • Restricted Access
  • Adobe PDF
  • 418.53 KB
Request a copy

Details

Authors
  • Debaetselier, A.
    Author
  • Dohet, P.
    Author
  • De Beukelaer, MichèleUCLouvain
    Author
  • Hathi, V.
    Author
  • Vasavada, A.
    Author
Show more
Abstract
The recombinant yeast expression plasmid, pSGO2, has been constructed containing a hybrid yeast alcohol dehydrogenase II-glyceraldehyde-3-phosphate dehydrogenase promoter, the glucose oxidase presequence and the mature glucose oxidase coding sequence. When transformed into yeast, this plasmid directs the synthesis and secretion of more than 1.5 g l-1 of active glucose oxidase. Analysis of the yeast-derived enzyme shows extensive N-linked glycosylation in comparison to the A. niger protein. Both proteins show similar specific activities in terms of U mg-1 of protein.
Affiliations

Citations

  • APA
  • Chicago
  • FWB

Debaetselier, A., Dohet, P., De Beukelaer, M., Hathi, V., Hanotier, J., Frederick, K., Rosenberg, S., & Vasavada, A. (1992). A New Production Method for Glucose-oxidase. Journal of Biotechnology, 24(2), 141-148. https://doi.org/10.1016/0168-1656(92)90117-R (Original work published 1992)