Evaluation of 2 In-111 Oxinate Formulations for Labeling of White Blood-cells
(1991) International Journal of Radiation Applications and Instrumentation. Part B: Nuclear Medicine and Biology — Vol. 18, n° 7, p. 727-& (1991)
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- This study compares the cell labelling characteristics of two In-111-oxinate formulations. The two preparations differ by the solubilizing agent of the chelate and the total amount of oxine. White blood cell suspensions were obtained by standard separation techniques and were labelled with either of these formulations. The labelling efficiency was higher for In-111-oxinate in aqueous solution (compound B) compared to the preparation where an organic solubilizer was added (compound A) (79.2 +/- 7.7 vs 68.6 +/- 17.6%, respectively, P = 0.03). Red blood cells contaminating the cell suspensions incorporated a higher fraction of In-111 if the cells were incubated with the aqueous In-111-oxinate preparation (22.6 +/- 4.6 vs 4.8 +/- 4.6%, respectively, P < 0.0001). The uptake of activity by polymorphonuclear cells was reduced with compound B (46.1 +/- 12%8 vs 63.8 +/- 15.8%, respectively, P = 0.0002) whereas the fraction retained by mononuclear cells and platelets was similar (31.3 +/- 13.9 vs 31.4 +/- 15.0%, respectively). The recovery from the vial was higher for In-111-oxinate in an organic solution (86.6 +/- 1.82 vs 60.3 +/- 14.3%, respectively, P < 0.0001). Twenty four hours after administration of the labelled cells, the vascular compartment was less frequently visualized if cells were labelled with compound A (8% of the scintigrams vs 62.5% respectively, P < 0.0001). High quality images were more often recorded after the administration of cells labelled with compound A (60.0% of the images vs 23.5%, respectively, P < 0.02). The image quality of scintigrams was not related to any of the other cell labelling parameters. We conclude that In-111-oxinate in an organic solubilizer was characterized by less uptake in the red blood cells contaminating the white blood cell suspensions. Good quality images were more often obtained with In-111-oxinate in organic solubilizer.
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Leners, N., Ferrant, A., & Jamar, F. (1991). Evaluation of 2 In-111 Oxinate Formulations for Labeling of White Blood-cells. International Journal of Radiation Applications and Instrumentation. Part B: Nuclear Medicine and Biology, 18(7), 727-&. https://hdl.handle.net/2078.5/137577 (Original work published 1991)