Combination of the mTOR inhibitor RAD001 with temozolomide and radiation effectively inhibits the growth of glioblastoma cells in culture

Burckel, Hélène;Josset, Elodie;Denis, Jean-Marc;Gueulette, John;Bischoff, Pierre L.;et.al.
(2015) Oncology Reports : an international journal devoted to fundamental and applied research in oncology — Vol. 33, n° 1, p. 471-477 (2015)

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Authors
  • Burckel, HélèneUniversité de Strasbourg, Radiobiology
    Author
  • Josset, ElodieUniversité de Strasbourg, Radiobiology
    Author
  • Denis, Jean-MarcUCLouvain
    Author
  • Gueulette, JohnUCLouvain
    Author
  • Bischoff, Pierre L.Université de Strasbourg
    Author
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Abstract
The present in vitro study aimed to assess the effects of combining the mTOR inhibitor RAD001 and temozolomide (TMZ) together with irradiation by either low-linear energy transfer (LET) radiation (γ-rays) or high-LET radiation (fast neutrons) on the growth and cell survival of the human glioblastoma cell line U-87. We observed a strong decrease in cell proliferation along with a concomitant increase in cell death as a function of the radiation dose. As expected, high-LET radiation was more effective and induced more sustained damage to DNA than low-LET radiation. While RAD001 in association with TMZ induced autophagic cell death, additional combination with either type of radiation did not further increase its occurrence. On the contrary, apoptosis remained at a low level in all experimental groups.
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Burckel, H., Josset, E., Denis, J.-M., Gueulette, J., Slabbert, J., Noël, G., & Bischoff, P. L. (2015). Combination of the mTOR inhibitor RAD001 with temozolomide and radiation effectively inhibits the growth of glioblastoma cells in culture. Oncology Reports : an international journal devoted to fundamental and applied research in oncology, 33(1), 471-477. https://doi.org/10.3892/or.2014.3590 (Original work published 2015)