Simultaneous Expression of Smooth and Rough Phase Properties Related To Lipopolysaccharide in a Strain of Brucella-melitensis
(1993) Journal of Medical Microbiology — Vol. 39, n° 5, p. 363-370 (1993)
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- Abstract
- Brucella strains exhibit either a rough (R) or a smooth (S) colonial phase identifiable by bacteriological methods. This depends on the biosynthesis and translocation to the surface in S but not in R strains, of the O-polysaccharide chain of the lipopolysaccharide (LPS) molecule. B. melitensis biovar 1 strain EP exhibited simultaneously both S and R characteristics in relation to colonial morphology, agglutination by monospecific anti-M and anti-R sera, activity of bacteriophages lytic for rough Brucella spp. (phage R/C) and for smooth B. melitensis (phage Iz). B. melitensis strain EP expressed fewer O-chains with a similar distribution of molecular weights than B. melitensis reference strain 16M by SDS-PAGE and immunoblotting, but higher amounts of R-LPS. Quantitative determination of S-LPS by a turbidimetric latex inhibition immunoassay with monoclonal antibodies confirmed the limited expression of S-LPS in strain EP. As with other gram-negative bacteria, the phenomenon could be attributed to a deficiency in one step of the biosynthetic assembly of the O-chains.
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Bowden, RA., Verger, JM., Grayon, M., Limet, JN., & Dubray, G. (1993). Simultaneous Expression of Smooth and Rough Phase Properties Related To Lipopolysaccharide in a Strain of Brucella-melitensis. Journal of Medical Microbiology, 39(5), 363-370. https://doi.org/10.1099/00222615-39-5-363 (Original work published 1993)