The tumor protein MAGE-1 is located in the cytosol of human melanoma cells
(1994) Biochemical and Biophysical Research Communications — Vol. 204, n° 2, p. 710-715 (1994)
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- The MAGE-1 protein has been localized to the cytosol of human melanoma culture cells (MZ2-MEL.43) by subcellular fractionation. Its distribution between nuclear, mitochondrial, microsomal and cytosolic fractions was established by SDS-PAGE and immunoblotting with a rabbit antiserum and compared to that of markers for the main cell compartments. The immunoreactive material was recovered in the cytosolic fraction as a polypeptide migrating at 42 kDa, which has been further identified as the MAGE-1 protein because it comigrates with the product of cell-free transcription-translation of the MAGE-1 cDNA. Location to the cytosol was confirmed by immunofluorescence microscopy.
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Citations
Amar-Costesec, A., Godelaine, D., Stockert, E., van der Bruggen, P., Beaufay, H., & Chen, Y.-T. (1994). The tumor protein MAGE-1 is located in the cytosol of human melanoma cells. Biochemical and Biophysical Research Communications, 204(2), 710-715. https://doi.org/10.1006/bbrc.1994.2517 (Original work published 1994)