Localization of 3'-nucleotidase and calcium-dependent endoribonuclease in the plasma-membrane of Trypanosoma brucei.
(1986) Acta Tropica — Vol. 43, n° 4, p. 295-305 (1986)
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- Abstract
- We have characterized a 3'-nucleotidase activity of T. brucei. The enzyme has a pH optimum of 8.7, is inactivated by chelating agents and stimulated by divalent cations. It is inhibited by Zn2+, Mn2+, pyrophosphate and the trypanocidal drug suramin for which it has a Ki of 3 microM. From cell fractionation experiments it is concluded that the enzyme is located in the plasma membrane. Alkaline 3'-endoribonuclease is also located in the plasma membrane of T. brucei and this activity shares a great number of properties with the 3'-nucleotidase activity, including its sensitivity to suramin. The possibility that both 3'-nucleotidase and endonuclease activities are catalyzed by the same enzyme cannot be excluded.
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Gbenle, G. O., Opperdoes, F., & Van Roy, J. (1986). Localization of 3′-nucleotidase and calcium-dependent endoribonuclease in the plasma-membrane of Trypanosoma brucei. Acta Tropica, 43(4), 295-305. https://hdl.handle.net/2078.5/134042 (Original work published 1986)