Fungal PDR transporters: Phylogeny, topology, motifs and function
(2010) Fungal Genetics and Biology — Vol. 47, n° 2, p. 127-142 (2010)
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- The overexpression of pleiotropic drug resistance (PDR) efflux pumps of the ATP-binding cassette (ABC) transporter superfamily frequently correlates with multidrug resistance. Phylogenetic analysis of 349 full-size (similar to 160 kDa) PDR proteins (Pdrps) from 55 fungal species, including major fungal pathogens, identified nine separate protein clusters (A-G, H1a/H1b and H2). Fungal, plant and human ABCG-family Pdrps possess a nucleotide-binding domain [NBD] and a transmembrane domain [TMD] in a family-defining 'reverse' ABC transporter topology [NBD-TMD] that is duplicated [NBD-TMD](2) in full-size fungal and plant Pdrps. Although full-size Pdrps have similar halves indicating early gene duplication/fusion, they show asymmetry of their NBDs and extracellular loops (ELs). Members of cluster F are most symmetric and may be closely related to the evolutionary ancestor of Pdrps. Unique structural elements are predicted, new PDR-specific motifs identified, and the significance of these and other structural features discussed. (c) 2009 Elsevier Inc. All rights reserved.
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Lamping, E., Baret, P., Holmes, A. R., Monk, B. C., Goffeau, A., & Cannon, R. D. (2010). Fungal PDR transporters: Phylogeny, topology, motifs and function. Fungal Genetics and Biology, 47(2), 127-142. https://doi.org/10.1016/j.fgb.2009.10.007 (Original work published 2010)