Porous reduced graphene oxide modified electrodes for the analysis of protein aggregation. Part 2: Application to the analysis of calcitonin containing pharmaceutical formulation

Vasilescu, Alina;Ye, Ran;Boulahneche, Samia;Lamraoui, Sabrina;Szunerits, Sabine;et.al.
(2018) Electrochimica Acta — Vol. 266, p. 364-372 (2018)

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Authors
  • Vasilescu, AlinaInternational Center of Biodynamics, Bucharest/Romania
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  • Ye, RanUCLouvain
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  • Boulahneche, SamiaUniversité de Lille, CNRS, Centrale Lille, ISEN, Université de Valenciennes, UMR 8520-IEMN, Lille/France
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  • Lamraoui, SabrinaUniversité de Lille, CNRS, Centrale Lille, ISEN, Université de Valenciennes, UMR 8520-IEMN, Lille/France
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  • Melinte, SorinUCLouvain
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  • Szunerits, SabineUniversité de Lille, CNRS, Centrale Lille, ISEN, Université de Valenciennes, UMR 8520-IEMN, Lille/France
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Abstract
In part 1 (A. Vasilescu et al., Porous reduced graphene oxide modified electrodes for the analysis of protein aggregation. Part 1: Lysozyme aggregation at pH 2 and 7.4 Electrochem. Acta, 254 (2017) 375-383) we proposed porous reduced graphene oxide coated glassy carbon electrode (GC/prGO) in combination with differential pulse voltammetry as a new analytical tool for aggregation studies of proteins. Lysozyme was used as a model to follow its aggregation by electrochemical means at pH 2 and pH 7.4, leading to the formation of amyloid and amorphous aggregates, respectively. Part 2 of this work widens the scope of this approach by investigating a biopharmaceutical product, as the aggregation of peptide based drugs affects their therapeutic activity and can induce allergic reactions in patients. The salmon polypeptide calcitonin (sCT) was chosen as an example of a bioactive peptide with limited pharmaceutical potential due to a tendency to form cytotoxic aggregates and amyloid fibrils. For practical applications, screen printed electrodes (SPE) and flexible electrodes (FE) modified with polydiallyldimethylammonium (PDDA) and prGO by using the layer-by-layer deposition technique have been dev
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Vasilescu, A., Ye, R., Boulahneche, S., Lamraoui, S., Jijie, R., Medjram, M. S., Gaspar, S., Singh, S. K., Kurungot, S., Melinte, S., Boukherroub, R., & Szunerits, S. (2018). Porous reduced graphene oxide modified electrodes for the analysis of protein aggregation. Part 2: Application to the analysis of calcitonin containing pharmaceutical formulation. Electrochimica Acta, 266, 364-372. https://doi.org/10.1016/j.electacta.2018.02.038 (Original work published 2018)