Measurement of beta 2-microglobulin in serum by a particle-enhanced nephelometric immunoassay.
(1991) European Journal of Clinical Chemistry and Clinical Biochemistry — Vol. 29, n° 6, p. 401-404 (1991)
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- Abstract
- A particle-enhanced immunoassay of beta 2-microglobulin in serum is described. It is based on the agglutination of complexes formed between the serum beta 2-microglobulin and latex particles coated with F(ab')2 fragments of polyclonal anti-beta 2-microglobulin antibodies. The analytical range of the method is 0.50 to 16 mg/l; it can be extended by appropriate dilution to 0.12 to 80 mg/l with good precision (CV less than 5% over the whole range). The accuracy and the precision are confirmed by a good correlation with radioimmunoassay (n = 123, r = 0.993). No error due to antigen excess was observed, even up to 292 mg/l. The main advantages of the method are its simplicity, its low cost per test and its high sensitivity (final dilution of the sample at 1/1200) with no known interference. The calibration curve is stable for at least 2 weeks.
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Citations
Lievens, M., Woestyn, S., De Nayer, P., & Collet-Cassart, D. (1991). Measurement of beta 2-microglobulin in serum by a particle-enhanced nephelometric immunoassay. European Journal of Clinical Chemistry and Clinical Biochemistry, 29(6), 401-404. https://hdl.handle.net/2078.5/72735 (Original work published 1991)