A specific defect in CD3 gamma-chain gene transcription results in loss of T-cell receptor/CD3 expression late after human immunodeficiency virus infection of a CD4+ T-cell line.

Willard-Gallo, Karen E;Van de Keere, Fabienne;Kettmann, Richard
(1990) Proceedings of the National Academy of Sciences of the United States of America — Vol. 87, n° 17, p. 6713-6717 (1990)

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Authors
  • Willard-Gallo, Karen EUCLouvain
    Author
  • Van de Keere, FabienneUCLouvain
    Author
  • Kettmann, RichardDepartment of Molecular Biology, Faculty of Agronomy, B-5800 Gembloux, Belgium
    Author
Abstract
Sequential effects on cellular protein expression following human immunodeficiency virus (type 1) infection of a CD4+ T-cell line in vitro were investigated. Events in the human interleukin 2-dependent helper T-cell line WE17/10 are similar in several respects to the clinical progression in acquired immunodeficiency syndrome. WE17/10 cell infection is characterized by an extended period during which viral replication occurs without accompanying cytotoxicity and with a maximum 30% decrease in surface CD4. Cellular protein expression generally remains unaffected during this first phase of infection. However, after 2-3 months, a severe defect in the expression of the T-cell receptor/CD3 complex both on the cell surface and inside the cell becomes apparent. Other cell membrane markers, such as CD2 and CD25, remain constant throughout the course of infection; after its initial decrease, CD4 remains at 70% of control values. Lack of surface expression of the TCR/CD3 complex is correlated with a specific defect in transcription of the CD3 gamma-chain gene.
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Willard-Gallo, K. E., Van de Keere, F., & Kettmann, R. (1990). A specific defect in CD3 gamma-chain gene transcription results in loss of T-cell receptor/CD3 expression late after human immunodeficiency virus infection of a CD4+ T-cell line. Proceedings of the National Academy of Sciences of the United States of America, 87(17), 6713-6717. https://doi.org/10.1073/pnas.87.17.6713 (Original work published 1990)