A short treatment with galactomannan GM-CT-01 corrects the functions of freshly isolated human tumor-infiltrating lymphocytes.

Demotte, Nathalie;Bigirimana, René;Wieërs, Grégoire;Stroobant, Vincent;van der Bruggen, Pierre;et.al.
(2014) Clinical cancer research : an official journal of the American Association for Cancer Research — Vol. 20, n° 7, p. 1823-1833 (2014)

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Authors
  • Demotte, NathalieUCLouvain
    Author
  • Bigirimana, RenéUSL-B
    Author
  • Wieërs, Grégoire
    Author
  • Stroobant, VincentUCLouvain
    Author
  • Squifflet, Jean-LucUCLouvain
    Author
  • Courtoy, Pierre JUCLouvain
    Author
  • van der Bruggen, PierreUCLouvain
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Abstract
Several galectins are released by tumor cells and macrophages and accumulate in the tumor microenvironment. Galectin-1 and -3 were found to bind to glycosylated receptors at the surface of tumor-infiltrating lymphocytes (TIL), forming glycoprotein-galectin lattices that could reduce the motility and therefore the functionality of surface molecules. In contrast to blood T cells, human TIL show defective IFN-γ secretion upon ex vivo stimulation. We have previously shown that extracellular galectin-3 participates in the impairment of TIL functions. Indeed, disruption of glycoprotein-galectin-3 lattices using anti-galectin-3 antibodies, or N-acetyllactosamine as a competing sugar, boosted cytokine secretion by TIL. Here we have tested a clinical grade galectin antagonist: GM-CT-01, a galactomannan obtained from guar gum reported to be safe in more than 50 patients with cancer. TIL were isolated from human tumor ascites, treated for 2 to 20 hours with galectin antagonists and tested for function. We found that GM-CT-01 boosts cytotoxicity of CD8(+) TIL and their IFN-γ secretion in a dose-dependent manner. Treating TIL obtained from patients with various cancers, during a few hours, resulted in an increased IFN-γ secretion in up to 80% of the samples. These observations pave the way for investigating the potential benefit of this galectin antagonist in patients with cancer, alone or combined with cancer vaccination, in order to correct in vivo impaired functions of TIL.
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Demotte, N., Bigirimana, R., Wieërs, G., Stroobant, V., Squifflet, J.-L., Carrasco, J., Thielemans, K., Baurain, J.-F., Van Der Smissen, P., Courtoy, P. J., & van der Bruggen, P. (2014). A short treatment with galactomannan GM-CT-01 corrects the functions of freshly isolated human tumor-infiltrating lymphocytes. Clinical cancer research : an official journal of the American Association for Cancer Research, 20(7), 1823-1833. https://doi.org/10.1158/1078-0432.CCR-13-2459 (Original work published 2014)