Steroid biotransforming strains designated Cochliobolus lunatus m118 and Curvularia lunata AT46 are both Curvularia lunata var lunata

Rozman, D;Hennebert, GL.;Kunej, T;Deckock, C;Komel, R
(1996) Mycotaxon — Vol. 59, p. 489-498 (1996)

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  • Rozman, D
    Author
  • Hennebert, GL.
    Author
  • Kunej, T
    Author
  • Deckock, C
    Author
  • Komel, R
    Author
Abstract
The two strains designated ''Cochliobolus lunatus m118'' (MUCL 38696) and ''Curvularia lunata AT46'' (MUCL 38697) are both important for production of corticosteroids. The strain Cochliobolus lunatus m118 developed mycelium only; no conidia nor ascomata were observed Curvularia lunata AT46 produced some conidia on current growth media and conditions. We present for the first time the successful conidiation of ''m118'' on the special growth media and light. Although the strains did not cross nor did with other MUCL Curvularia lunata strains, remaining both asexual, they produced identical conidiation on specific media and under near UV light. Both strains gave the same results with API ZYM 20 (Biomerieux) test and they hybridized well with a randomly cloned Cochliobolus lunatus genomic probe. RAPD profiles with different primers showed some identical (species specific) markers and several nonidentical (strain specific) markers. Results demonstate that strains m118 and AT46 which have been widely used in biochemical and molecular biology studies but did not have a documented taxonomical classification, are two non-compatible Curvularia lunata var. lunata strains.
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Rozman, D., Hennebert, GL., Kunej, T., Deckock, C., & Komel, R. (1996). Steroid biotransforming strains designated Cochliobolus lunatus m118 and Curvularia lunata AT46 are both Curvularia lunata var lunata. Mycotaxon, 59, 489-498. https://hdl.handle.net/2078.5/69379 (Original work published 1996)