Nano-emulsions of fluorinated trityl radicals as sensors for EPR oximetry
(2009) Journal of Magnetic Resonance — Vol. 197, n° 2, p. 176-180 (2009)
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- Abstract
- This article reports the development and evaluation of two nano-emulsions (F45T-03/HFB and F15T-03/PFOB) containing fluorinated trityl radicals dissolved in perfluorocarbons. Preparation with a high-pressure homogenizer conferred sub-micronic size to both nano-emulsions. In vitro and in vivo EPR spectroscopy showed that the nano-emulsions had much greater oxygen sensitivity than the hydrophilic trityl, CT-03. In vivo experiments in rodents confirmed the ability of the nano-emulsions to follow the changes in oxygen concentration after induced ischemia. Histological evaluation of the tissue injected with the nano-emulsions revealed some acute toxicity for the F45T-03/HFB nano-emulsion but none for the F15T-03/PFOB nano-emulsion. These new formulations should be considered for further EPR oximetry experiments in pathophysiological situations where subtle changes in tissue oxygenation are expected.
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Citations
Charlier, N., Driesschaert, B., Wauthoz, N., Beghein, N., Préat, V., Amighi, K., Marchand-Brynaert, J., & Gallez, B. (2009). Nano-emulsions of fluorinated trityl radicals as sensors for EPR oximetry. Journal of Magnetic Resonance, 197(2), 176-180. https://doi.org/10.1016/j.jmr.2008.12.013 (Original work published 2009)