Sample preparation procedures for biological atomic force microscopy.

El Kirat, Karim;Burton, I;Dupres, Vincent;Dufrêne, Yves
(2005) Journal of Microscopy — Vol. 218, n° Pt 3, p. 199-207 (2005)

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Authors
  • El Kirat, KarimUCLouvain
    Author
  • Burton, IUCLouvain
    Author
  • Dupres, VincentUCLouvain
    Author
  • Dufrêne, YvesUCLouvain
    Author
Abstract
Since the late 1980s, atomic force microscopy (AFM) has been increasingly used in biological sciences and it is now established as a versatile tool to address the structure, properties and functions of biological specimens. AFM is unique in that it provides three-dimensional images of biological structures, including biomolecules, lipid films, 2D protein crystals and cells, under physiological conditions and with unprecedented resolution. A crucial prerequisite for successful, reliable biological AFM is that the samples need to be well attached to a solid substrate using appropriate, nondestructive methods. In this review, we discuss common techniques for immobilizing biological specimens for AFM studies.
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El Kirat, K., Burton, I., Dupres, V., & Dufrêne, Y. (2005). Sample preparation procedures for biological atomic force microscopy. Journal of Microscopy, 218(Pt 3), 199-207. https://doi.org/10.1111/j.1365-2818.2005.01480.x (Original work published 2005)